Prime Editing Mechanisms: Precise Gene Rewriting Without RNA Templates
How prime editing encodes precise genomic changes into a single guide RNA—no templates, no double-strand breaks
Transposon Tools: Genomic Engineering at Random and Targeted Sites
How engineered transposases turn ancient jumping genes into precision tools for genomics and cell therapy
Feedback Control in Genetic Circuits: Achieving Robust Performance
Control theory meets molecular biology: engineering genetic circuits that hold steady when cells won't
Biosafety Engineering: Designing Containment into Synthetic Organisms
How synthetic biologists engineer organisms that cannot survive outside their intended environment
Quorum Sensing Circuits: Engineering Population-Level Communication
How engineered bacterial communication enables coordinated behaviors impossible for individual cells to achieve alone
Secretion Pathway Engineering: Getting Products Out of the Cell
Engineering cellular export machinery to simplify purification and enable continuous bioprocessing
Promoter Engineering: Tuning Gene Expression with Quantitative Precision
Design rules for constructing synthetic promoters with predictable transcriptional outputs across orders of magnitude
AAV Vector Engineering: Designing the Next Generation of Gene Therapy Vehicles
How systematic capsid and genome engineering is creating delivery vehicles that outperform anything evolution provided
Protein Degradation Control: Engineering Turnover for Dynamic Regulation
Controlled protein destruction enables regulatory precision that transcription alone cannot achieve
Chassis Cell Selection: Matching Host Biology to Production Requirements
A systematic framework for matching expression platforms to the metabolic, processing, and economic requirements of biotechnology products.
Strain Stability: Preventing Genetic Drift in Production Organisms
Engineering production organisms that evolution cannot easily undo
Directed Evolution Strategies: Accelerating Enzyme Engineering Without Structural Knowledge
When you can't predict which mutations will work, let evolution show you the answer.
Base Editing Precision: Beyond Double-Strand Breaks in Genome Engineering
Chemical nucleotide conversion offers safer genome modification by keeping DNA intact while rewriting genetic letters.
Cell-Free Systems: Engineering Biosynthesis Without the Cell
Strip away the cell membrane and gain direct control over biological machinery for faster prototyping, toxic product synthesis, and deployable diagnostics.
CRISPR Delivery Engineering: Getting Gene Editors Where They Need to Go
Why the most elegant gene editing designs fail without delivery systems engineered to navigate biological barriers and reach target cells with precision.
Biosensor Design: Engineering Cells That Detect and Report Molecular Signals
Master the systematic engineering of living cells into programmable molecular detection platforms for diagnostics and monitoring applications.
Bioprocess Scale-Up: Why Lab Success Often Fails at Production Scale
Master the physics of large bioreactors before your cells discover the difference the hard way.
Codon Optimization Beyond the Basics: Why Standard Tables Fail and What Actually Works
Master the translational dynamics, mRNA structures, and context-dependent factors that determine whether your codon-optimized sequences actually express functional protein.
Engineering Living Circuits: How Biological Toggle Switches Transform Cellular Programming
Master the foundational memory circuits that enable cells to compute, remember, and respond with digital precision in programmable biological systems.
Metabolic Flux Analysis Demystified: Mapping the Flow of Carbon Through Engineered Pathways
Master the quantitative framework that reveals where carbon actually flows in your engineered pathways and how to redirect it toward maximum yields.
Riboswitch Engineering: RNA-Based Control Without Protein Regulators
Design compact RNA-based gene switches that respond to small molecules without requiring additional protein machinery or genetic burden.